cotG

168

outer spore coat protein

Locus: BSU_36070

Molecular weight: 23.81 kDa

Isoelectric point: 10.67

Protein length: 195 aa

Gene length: 588 bp

Function: control of spore permeability, resistance of the spore

Product: spore coat protein

Essential: no

Synonyms: cotG

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,717,238  3,717,825

The protein

Catalyzed reaction/ biological activity

• required for the maturation of CotB PubMed

Structure

• P39801 (AlphaFold)

Modification

• phosphorylated on Ser-15, Ser-39, and Thr-147 by CotH, phosphorylation does not take place at increased temperature PubMed

Localization

• outer spore coat, localization depends on CotE  PubMed
• assembly around the forming spore depends on CotH-mediated phosphorylation PubMed
• accumulation In the mother cell (non-phosphorylated CotG) PubMed

Expression and Regulation

Operons

• Genes: cotG

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon
  • GerR: activation, PubMed, in gerR regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE36070 (cotG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTGAAATCCTCCTTTT,  downstream forward: _UP4_TAATCTAAGTTTTCATTGTT
• BKK36070 (cotG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTGAAATCCTCCTTTT,  downstream forward: _UP4_TAATCTAAGTTTTCATTGTT

References

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