dhbF

168

involved in 2,3-dihydroxybenzoate biosynthesis

Locus: BSU_31960

Molecular weight: 263.44 kDa

Isoelectric point: 4.74

Protein length: 2378 aa

Gene length: 7137 bp

Function: biosynthesis of the siderophore bacillibactin

Product: unknown

Essential: no

Synonyms: dhbF

Genomic Context

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List of homologs in different organisms, belongs to COG3319 (Galperin et al., 2021)

Gene

Coordinates: 3,280,519  3,287,655

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Domains

• 2 Carrier domains (aa 961-1036, aa 2036-2111) (according to UniProt)

Structure

• 2VSQ (PDB) (termination module of SrfAC, 31% identity) PubMed
• P45745 (AlphaFold)

Modification

• phosphorylation on Ser-988 AND Ser-996 PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ

  Description: PubMed

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression is increased in the presence of pulchimerrin PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon
  • AbrB: repression, PubMed, in abrB regulon
  • Kre: repression, in kre regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigI: sigma factor, PubMed, in sigI regulon

  Additional information

  • the ''dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre'' mutant PubMed

Biological materials

Mutant

• MGNA-A063 (yukL::erm), available at the NBRP B. subtilis, Japan
• BKE31960 (dhbF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC,  downstream forward: _UP4_AATAAATAAGGGAGGAGAAA
• BKK31960 (dhbF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC,  downstream forward: _UP4_AATAAATAAGGGAGGAGAAA

References

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