dhbE

168

2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)

Locus: BSU_31980

Molecular weight: 59.76 kDa

Isoelectric point: 5.68

Protein length: 539 aa

Gene length: 1620 bp

Function: biosynthesis of the siderophore bacillibactin

Product: 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)

Essential: no

Synonyms: dhbE, entE

Genomic Context

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List of homologs in different organisms, belongs to COG1021 (Galperin et al., 2021)

Gene

Coordinates: 3,288,641 → 3,290,260

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Structure

• 1MDB (PDB) (complex with DHB-adenylate),  1MDF (PDB)
• P40871 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ

  Description: PubMed

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression is increased in the presence of pulchimerrin PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon
  • AbrB: repression, PubMed, in abrB regulon
  • Kre: repression, in kre regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigI: sigma factor, PubMed, in sigI regulon

  Additional information

  • the ''dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre'' mutant PubMed

Biological materials

Mutant

• BKE31980 (ΔdhbE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTATCCCTCCAGCA,  downstream forward: _UP4_TAAAACAACAATTTGAGAGG
• BKK31980 (ΔdhbE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTATCCCTCCAGCA,  downstream forward: _UP4_TAAAACAACAATTTGAGAGG

Labs working on this gene/protein

• Mohamed Marahiel, Marburg University, Germany homepage

References

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