folEB

168

GTP cyclohydrolase IB, required for the initial step of queuosine synthesis (tRNA modification), replaces FolE under conditions of zinc starvation

Locus: BSU_03340

Molecular weight: 34.63 kDa

Isoelectric point: 7.83

Protein length: 305 aa

Gene length: 915 bp

Function: biosynthesis of folate and queuosine

Product: zinc-independent GTP cyclohydrolase IB

Essential: no

E.C.: 3.5.4.16

Synonyms: folEB, folE2, yciA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1469 (Galperin et al., 2021)

Gene

Coordinates: 364,259 → 365,173

The protein

Catalyzed reaction/ biological activity

• GTP + H₂O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H⁺ (according to UniProt)

Protein family

• GTP cyclohydrolase IV family (single member, according to UniProt)

Cofactors

• activated by a variety of divalent cations PubMed

Structure

• 3D1T (PDB) PubMed
• P94398 (AlphaFold)

Expression and Regulation

Operons

• Genes: folEB-yciB-yczL-zagA

  Description: PubMed

  Regulation

  • folE2: induced by zinc starvation (Zur) PubMed

  Regulatory mechanism

  • Zur: repression, in the presence of zinc PubMed, in zur regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE03340 (ΔfolEB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA,  downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
• BKK03340 (ΔfolEB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA,  downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA

References

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