sigB

168

RNA polymerase sigma factor SigB

Locus: BSU_04730

Molecular weight: 29.99 kDa

Isoelectric point: 5.42

Protein length: 262 aa

Gene length: 789 bp

Function: general stress response, biocontrol of fungal growth

Product: RNA polymerase sigma factor SigB

Essential: no

Synonyms: sigB, rpoF

Genomic Context

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List of homologs in different organisms, belongs to COG5812 (Galperin et al., 2021)

Gene

Coordinates: 522,862  523,650

The protein

Protein family

• Sigma-70 factor family (according to UniProt)

Structure

• 1L0O (PDB) (SigF-SpoIIAB complex from Geobacillus stearothermophilus, 32% identity) PubMed
• P06574 (AlphaFold)

Effectors of protein activity

• SigB is activated during bacterial-fungal interaction (via RsbP) PubMed

Expression and Regulation

Operons

• Genes: rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX

  Description: PubMed

  Regulation

  • ''rsbV:'' induced by stress (SigB) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: rsbV-rsbW-sigB-rsbX

  Description: PubMed

  Regulation

  • ''rsbV:'' induced by stress (SigB) PubMed

Biological materials

Mutant

• QB5344 (cat), available in Jörg Stülke's lab
• 1A675 ( sigB::cat), PubMed, available at BGSC
• 1A777 ( sigB::cat), PubMed, available at BGSC
• 1A780 ( sigB::spec), PubMed, available at BGSC
• BKE04730 (sigB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTTATGAGCCGATCGACTT,  downstream forward: _UP4_GATCCCTCGATGGAGTTAAT
• BKK04730 (sigB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTTATGAGCCGATCGACTT,  downstream forward: _UP4_GATCCCTCGATGGAGTTAAT

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Reviews

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Other publications

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Identification of the SigB regulon

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Control of SigB activity by protein-protein interactions

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