RpsJ

• Description: ribosomal protein
  
  

Gene name	rpsJ
Synonyms	tetA
Essential	yes PubMed
Product	ribosomal protein S10 (BS13)
Function	translation
Gene expression levels in SubtiExpress: rpsJ
Interactions involving this protein in SubtInteract: RpsJ
MW, pI	11 kDa, 10.207
Gene length, protein length	306 bp, 102 aa
Immediate neighbours	ybaC, rplC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01150

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01150

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S10P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • located near the aminoacyl-tRNA site of the 30S ribosomal subunit PubMed

Database entries

• BsubCyc: BSU01150

• Structure:

• UniProt: P21471

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO PubMed

• Expression browser: rpsJ PubMed

• Sigma factor:
  • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 8159 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 13952 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:
  • pGP605 (in pAC6), available in Jörg Stülke's lab
  • pGP2201 (in pAC7), available in Jörg Stülke's lab
  • pGP2439 (in pAC7), available in Jörg Stülke's lab
  • pGP2472 (in pAC5), available in Jörg Stülke's lab
  • pGP2473 (in pAC6), available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tom Coenye, Peter Vandamme
Organisation of the S10, spc and alpha ribosomal protein gene clusters in prokaryotic genomes.
FEMS Microbiol Lett: 2005, 242(1);117-26
[PubMed:15621428] [WorldCat.org] [DOI] (P p)

Original publications

Genki Akanuma, Shota Suzuki, Koichi Yano, Hideaki Nanamiya, Yousuke Natori, Eri Namba, Kazuya Watanabe, Kazumi Tagami, Takuya Takeda, Yuka Iizuka, Ako Kobayashi, Morio Ishizuka, Hirofumi Yoshikawa, Fujio Kawamura
Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis.
J Gen Appl Microbiol: 2013, 59(2);105-17
[PubMed:23759864] [WorldCat.org] [DOI] (I p)

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

F Schluenzen, A Tocilj, R Zarivach, J Harms, M Gluehmann, D Janell, A Bashan, H Bartels, I Agmon, F Franceschi, A Yonath
Structure of functionally activated small ribosomal subunit at 3.3 angstroms resolution.
Cell: 2000, 102(5);615-23
[PubMed:11007480] [WorldCat.org] [DOI] (P p)

X Li, L Lindahl, Y Sha, J M Zengel
Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster identifies two promoters that may be responsible for transcription of the entire 15-kilobase S10-spc-alpha cluster.
J Bacteriol: 1997, 179(22);7046-54
[PubMed:9371452] [WorldCat.org] [DOI] (P p)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)