mtrB

168

tryptophan operon RNA-binding attenuation protein (TRAP), controls RNA switch in front of genes involved in biosynthesis and acquisition of tryptophan

Locus: BSU_22770

Molecular weight: 8.19 kDa

Isoelectric point: 7.33

Protein length: 75 aa

Gene length: 228 bp

Function: regulation of tryptophan biosynthesis(and translation) attenuation in the trp operon;repression of the folate operon

Product: tryptophan operon RNA-binding attenuation protein (TRAP)

Essential: no

Synonyms: mtrB

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,384,534 → 2,384,761

The protein

Protein family

• MtrB family (single member, according to UniProt)

Structure

• 3AQD (PDB),  1WAP (PDB) (complex with L-tryptophan), 2ZP9 (PDB) (complex with RtpA)
• P19466 (AlphaFold)

Effectors of protein activity

• binding of tryptophan results RNA binding and thus in transcription termination

Expression and Regulation

Operons

• Genes: folE-mtrB

  Description: PubMed

  Regulation

  • constitutive PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE22770 (ΔmtrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATAGCACCCTCTATC,  downstream forward: _UP4_TAAGCTGGCTGTCCCCGCTG
• BKK22770 (ΔmtrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATAGCACCCTCTATC,  downstream forward: _UP4_TAAGCTGGCTGTCCCCGCTG

References

Reviews

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Original Publications

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