bcd

168

valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase

Locus: BSU_24080

Molecular weight: 39.83 kDa

Isoelectric point: 4.94

Protein length: 364 aa

Gene length: 1095 bp

Function: utilization of branched-chain keto acids

Product: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase

Essential: no

E.C.: 1.4.1.9

Synonyms: bcd, bkd, yqiT

Genomic Context

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List of homologs in different organisms, belongs to COG0334 (Galperin et al., 2021)

Gene

Coordinates: 2,502,659  2,503,753

Phenotypes of a mutant

• premature spore germination due to valine accumulation, activation of germination via GerAA-GerAB-GerAC PubMed

The protein

Catalyzed reaction/ biological activity

• L-leucine + H₂O + NAD⁺ --> 4-methyl-2-oxopentanoate + NH₄⁺ + NADH + H⁺ (according to UniProt)

Protein family

• Glu/Leu/Phe/Val dehydrogenases family (with GudB and RocG, according to UniProt)

Structure

• 1LEH (PDB) (from Lysinibacillus sphaericus, 71% identity, 82% similarity) PubMed
• P54531 (AlphaFold)

Expression and Regulation

Operons

• Genes: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

  Description: PubMed

  Regulation

  • induced in the presence of isoleucine or valine (BkdR) PubMed

  Regulatory mechanism

  • BkdR: activation, PubMed, in bkdR regulon
  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

• GP4802 (bcd::neo), available in Jörg Stülke's lab
• GP4803 (bcd::lox72), available in Jörg Stülke's lab
• MGNA-C427 (yqiT::erm), available at the NBRP B. subtilis, Japan
• a bcd mutant and a ''bcd ilvE ilvK'' triple mutant are available in Linc Sonenshein's lab
• BKE24080 (bcd::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAAAGTTCCATGTTCGTTC,  downstream forward: _UP4_CGTTAAGAAATTGATCTGGA
• BKK24080 (bcd::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAAAGTTCCATGTTCGTTC,  downstream forward: _UP4_CGTTAAGAAATTGATCTGGA

References

Reviews

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Original Publications

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