FlhF

• Description: signal recognition particle-like GTPase, placement and assembly of flagella, control of basal body position
  
  

Gene name	flhF
Synonyms
Essential	no
Product	signal recognition particle-like GTPase
Function	placement and assembly of polar flagella
Gene expression levels in SubtiExpress: flhF
Interactions involving this protein in SubtInteract: FlhF
MW, pI	40 kDa, 8.719
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	flhA, flhG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16400

Database entries

• BsubCyc: BSU16400

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • GTPase activity PubMed
  • placement of the first flagellar protein, FliF PubMed

• Protein family: GTP-binding SRP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • interaction with FlhG activates the GTPase activity of FlhF PubMed

• Interactions:
  • FlhG-FlhF (FlhG binds the GTP-bound homodimer of FlhF) PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16400

• Structure:
  • 2PX3 (complexed with GTP-Mg2+) PubMed
  • 3SYN (complex with its activator YlxH) PubMed

• UniProt: Q01960

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: flhF PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed
  • DegU-P: transcription repression PubMed
  • DegU-P in complex with SwrA: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 61 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 111 PubMed

Biological materials

• Mutant:
  • 1A925 ( flhF::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: