fabL

168

enoyl-acyl carrier protein reductase

locus: BSU_08650

Molecular weight: 27.03 kDa

pI: 5.97

Protein length: 250 aa

Gene length: 753 bp

function: fatty acid biosynthesis

product: enoyl-acyl carrier protein reductase

essential: no

synonyms: fabL, yfhR, ygaA

List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

Gene

Coordinates: 937,079  937,831

The protein

Catalyzed reaction/ biological activity

• 2,3-saturated acyl-[ACP] + NADP⁺ --> (2E)-enoyl-[ACP] + H⁺ + NADPH (according to UniProt)

Protein family

• [wiki|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Structure

• [PDB|3OIC] [Pubmed|21185310]
• [AF|P71079]

Paralogous protein(s)

• [protein|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI], one of the two proteins has to be present for viability [Pubmed|17114254]
  [protein|B6FF689E65906186F3576B378650D713DB84EDDA|ycdF], (31.6%)
  [protein|EAEEA4DD9641919830A81185333A2610B964D37C|yhdF], (30.1%)
  [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|ykvO], (31,8%)

Expression and Regulation

Operons

• genes: [gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]-[gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]

  description: [Pubmed|10463184]

  regulation

  • repressed by casamino acids [Pubmed|12107147]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
  • [protein|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|yfhP]: repression, in [regulon|protein:BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|yfhP regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10463184], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

• genes: [gene|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY]-[gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]-[gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]

  description: [Pubmed|10463184]

  regulation

  • expression is constitutive throughout growth [Pubmed|20971907]

  regulatory mechanism

  • [protein|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|yfhP]: repression, in [regulon|protein:BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|yfhP regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10463184], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|1900507], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-C324 (yfhR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2322 NBRP B. subtilis, Japan]
• BKE08650 ([gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCATCTCCTTG,  downstream forward: _UP4_TAAAAATTTTTAAAAAAGAG
• BKK08650 ([gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCATCTCCTTG,  downstream forward: _UP4_TAAAAATTTTTAAAAAAGAG

References

Reviews

• 15952903,17919287

Original Publications

• 17114254,10463184,15699190,8755877,11007778,21185310,25527535,36815589