arsC

168

arsenate reductase

Locus: BSU_25780

Molecular weight: 15.45 kDa

Isoelectric point: 4.75

Protein length: 139 aa

Gene length: 420 bp

Function: detoxification of arsenate

Product: arsenate reductase

Essential: no

E.C.: 3.1.3.48

Synonyms: arsC, yqcM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0394 (Galperin et al., 2021)

Gene

Coordinates: 2,655,322 → 2,655,741

The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-dithiol + arsenate + H⁺ --> [thioredoxin]-disulfide + arsenite + H₂O (according to UniProt)

Protein family

• low molecular weight phosphotyrosine protein phosphatase family (with YfkJ and YwlE, according to UniProt)

Structure

• 1Z2D (PDB) (reduced form),  1Z2E (PDB) (oxidized form),  2IPA (PDB) (TrxA-ArsC-complex) PubMed,  1JL3 (PDB)
• P45947 (AlphaFold)

Expression and Regulation

Operons

• Genes: arsR-yqcK-arsB-arsC

  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Regulatory mechanism

  • ArsR: repression, PubMed, in arsR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigF: sigma factor, PubMed, in sigF regulon

Biological materials

Mutant

• BKE25780 (ΔarsC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATTCCACCTCTACAT,  downstream forward: _UP4_TAAATAAACTCTTTTGTTAA
• BKK25780 (ΔarsC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATTCCACCTCTACAT,  downstream forward: _UP4_TAAATAAACTCTTTTGTTAA

References

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