YpjD

• Description: putative pyrophosphatase
  
  

Gene name	ypjD
Synonyms	jojD
Essential	no
Product	putative pyrophosphatase
Function	unknown
Gene expression levels in SubtiExpress: ypjD
Metabolic function and regulation of this protein in SubtiPathways: ypjD
MW, pI	12 kDa, 4.952
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	dapB, ypjC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU22500

Database entries

• BsubCyc: BSU22500

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU22500

• Structure: 2GTA

• UniProt: P42979

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: ypjD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by diamide stess (thiol depletion) (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:
  • pGP2437 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Operon and expression

Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)