yvrB

168

ABC transporter (membrane protein) for cobalamin

Locus: BSU_33170

Molecular weight: 37.34 kDa

Isoelectric point: 11.07

Protein length: 353 aa

Gene length: 1062 bp

Function: uptake of cobalamin

Product: ABC transporter (membrane domain)

Essential: no

Synonyms: yvrB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0609 (Galperin et al., 2021)

Gene

Coordinates: 3,402,469  3,403,530

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• FecCD subfamily (according to UniProt)

Structure

• 4R9U (PDB), the E. coli BtuC-BtuD complex, BtuC shares 40% identity, 71% similarity with YvrB, PubMed
• O34451 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yvrC-yvrB-yvrA-yvqK

  Regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) (B12 riboswitch) PubMed

  Regulatory mechanism

  • B12 riboswitch: RNA switch, upon binding of B12 PubMed, in B12 riboswitch

Biological materials

Mutant

• MGNA-B044 (yvrB::erm), available at the NBRP B. subtilis, Japan
• BKE33170 (yvrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACCAATGATATATGCGGCAT,  downstream forward: _UP4_CATCGCGGGGGGAGAAGCTT
• BKK33170 (yvrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACCAATGATATATGCGGCAT,  downstream forward: _UP4_CATCGCGGGGGGAGAAGCTT

References

Loading