fhuC

168

hydroxamate siderophore ABC transporter (ATP-binding protein) (ferrichrome und ferrioxamine)

Locus: BSU_33290

Molecular weight: 29.80 kDa

Isoelectric point: 6.5

Protein length: 269 aa

Gene length: 810 bp

Function: siderophore uptake

Product: hydroxamate siderophore ABC transporter (ATP-binding protein) (ferrichrome und ferrioxamine)

Essential: no

E.C.: 3.6.3.34

Synonyms: fhuC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1120 (Galperin et al., 2021)

Gene

Coordinates: 3,415,387 → 3,416,196

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + Fe³⁺-hydroxamate complex-[hydroxamate-binding protein](Side 1) --> ADP + phosphate + Fe³⁺-hydroxamate complex(Side 2) + [hydroxamate-binding protein] (according to UniProt)

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 4-240) (according to UniProt)

Structure

• 4R9U (PDB), the E. coli BtuC-BtuD complex, BtuD shares 34% identity, 55% similarity with FhuC, PubMed
• P49938 (AlphaFold)

Paralogous protein(s)

• FecF, YusV, FpbP

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: fhuB-fhuG-fhuC

  Description

  • PubMed

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

Biological materials

Mutant

• BKE33290 (ΔfhuC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGGCCCCCTCCTCT,  downstream forward: _UP4_TGAAAAAATCCCGGTTTGAA
• BKK33290 (ΔfhuC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGGCCCCCTCCTCT,  downstream forward: _UP4_TGAAAAAATCCCGGTTTGAA

References

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