sigH

168

RNA polymerase sigma factor SigH, not fully active in laboratory strains due to a mutation (V117A)

Locus: BSU_00980

Molecular weight: 25.30 kDa

Isoelectric point: 5.15

Protein length: 218 aa

Gene length: 657 bp

Function: transcription of early stationary phase genes (sporulation, competence)

Product: RNA polymerase sigma factor SigH

Essential: no

Synonyms: sigH, spo0H

Genomic Context

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Categories containing this gene/protein

Information processing, RNA synthesis and degradation, Transcription, Sigma factors

Information processing, Regulation of gene expression, Sigma factors and their control, Sigma factors

List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, AbrB regulon, SigH regulon

Gene

Coordinates: 116,600 → 117,256

The protein

Protein family

Sigma-70 factor family (according to UniProt)

Domains

HTH luxR-type domain (aa 179-206) (according to InterPro)

Structure

3MZY (PDB) (from Fusobacterium nucleatum, 40% identity)
P17869 (AlphaFold)

Additional information

not fully active in laboratory strains due to a mutation (V117A) PubMed
the poor interaction of SigH with the RNA polymerase is responsible for the poor sporulation at high salinity, this phenotype can be partially suppressed by specific mutations in SigH PubMed

Expression and Regulation

Operons

Genes: sigH-rpmGB-secE

Description: PubMed

Regulation

repressed during logrithmic growth (AbrB) PubMed

Regulatory mechanism

AbrB: repression, PubMed, in abrB regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

BKE00980 (ΔsigH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC,  downstream forward: _UP4_TAATAGGAATTTATGCTATA
BKK00980 (ΔsigH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC,  downstream forward: _UP4_TAATAGGAATTTATGCTATA

Labs working on this gene/protein

Charles Moran, Emory University, NC, USA homepage

References

Reviews

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Original Publications

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The SigH regulon

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