sigH

168

RNA polymerase sigma factor SigH, not fully active in laboratory strains due to a mutation (V117A)

Locus: BSU_00980

Molecular weight: 25.30 kDa

Isoelectric point: 5.15

Protein length: 218 aa

Gene length: 657 bp

Function: transcription of early stationary phase genes (sporulation, competence)

Product: RNA polymerase sigma factor SigH

Essential: no

Synonyms: sigH, spo0H

Genomic Context

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List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

Gene

Coordinates: 116,600 → 117,256

The protein

Protein family

• Sigma-70 factor family (according to UniProt)

Domains

• HTH luxR-type domain (aa 179-206) (according to InterPro)

Structure

• 3MZY (PDB) (from Fusobacterium nucleatum, 40% identity)
• P17869 (AlphaFold)

Additional information

• not fully active in laboratory strains due to a mutation (V117A) PubMed
• the poor interaction of SigH with the RNA polymerase is responsible for the poor sporulation at high salinity, this phenotype can be partially suppressed by specific mutations in SigH PubMed

Expression and Regulation

Operons

• Genes: sigH-rpmGB-secE

  Description: PubMed

  Regulation

  • repressed during logrithmic growth (AbrB) PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE00980 (ΔsigH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC,  downstream forward: _UP4_TAATAGGAATTTATGCTATA
• BKK00980 (ΔsigH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC,  downstream forward: _UP4_TAATAGGAATTTATGCTATA

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

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Original Publications

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The SigH regulon

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