hepT

168

heptaprenyl diphosphate synthase component II

Locus: BSU_22740

Molecular weight: 39.36 kDa

Isoelectric point: 6.28

Protein length: 348 aa

Gene length: 1047 bp

Function: menaquinone biosynthesis

Product: heptaprenyl diphosphate synthase component II (together with HepS)

Essential: no

E.C.: 2.5.1.30

Synonyms: hepT, gerCC, hepB, gerC58

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0142 (Galperin et al., 2021)

Gene

Coordinates: 2,381,919  2,382,965

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed

The protein

Catalyzed reaction/ biological activity

• (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate --> all-trans-heptaprenyl diphosphate + 4 diphosphate (according to UniProt)

Protein family

• FPP/GGPP synthase family (with YqiD, according to UniProt)

Structure

• 5H9D (PDB) (from Staphylococcus aureus, 47% identity) PubMed
• P31114 (AlphaFold)

Paralogous protein(s)

• YqiD

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hepS-menH-hepT-ndk

  Description: PubMed

Biological materials

Mutant

• BKE22740 (hepT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCATTCACCCAAACATCTG,  downstream forward: _UP4_TAATTTTTGTAGATATTAAG
• BKK22740 (hepT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCATTCACCCAAACATCTG,  downstream forward: _UP4_TAATTTTTGTAGATATTAAG

References

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