gerAC

168

lipoprotein, nutrient receptor, germination response to L-alanine, triggers premature germination in response to morphological defects during sporulation

Locus: BSU_33070

Molecular weight: 42.23 kDa

Isoelectric point: 7.3

Protein length: 373 aa

Gene length: 1122 bp

Function: germination response to L-alanine

Product: part of the nutrient-gated GerAA-GerAB-GerAC ion channel

Essential: no

Synonyms: gerAC

Genomic Context

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List of homologs in different organisms, belongs to COG5903 (Galperin et al., 2021)

Gene

Coordinates: 3,393,293 → 3,394,414

Phenotypes of a mutant

• suppresses the defects of spoVV mutant spores PubMed

The protein

Catalyzed reaction/ biological activity

• the GerAA-GerAB-GerAC ion channel releases ions from the spore core in response to L-alanine to trigger Germination PubMed
• triggers premature germination in response to morphological defects during sporulation PubMed

Protein family

• GerABKC lipoprotein family (according to UniProt)

Domains

• has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide PubMed

Structure

• 3N54 (PDB) (GerBC, 34% identity) PubMed
• P07870 (AlphaFold)

Paralogous protein(s)

• GerBC

Localization

• cell membrane (according to UniProt)
• outer surface of the inner spore membrane PubMed

Additional information

• 1,100 molecules are present per spore PubMed

Expression and Regulation

Operons

• Genes: gerAA-gerAB-gerAC

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: repression, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon
  • SigF: sigma factor, PubMed, in sigF regulon

  Additional information

  • 1,100 molecules are present per spore PubMed

Biological materials

Mutant

• BKE33070 (ΔgerAC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACATACATAAAATCCGGA,  downstream forward: _UP4_AAAGGCGCAAACAAATAGCA
• BKK33070 (ΔgerAC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACATACATAAAATCCGGA,  downstream forward: _UP4_AAAGGCGCAAACAAATAGCA

References

Reviews

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Original Publications

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