ypeP

168

similar to RNase HI

Locus: BSU_21970

Molecular weight: 13.58 kDa

Isoelectric point: 5.24

Protein length: 226 aa

Gene length: 681 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ypeP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0328 (Galperin et al., 2021)

Gene

Coordinates: 2,308,967  2,309,647

The protein

Domains

• RNase H domain (aa 71-207) (according to UniProt)

Structure

• P54164 (AlphaFold)

Expression and Regulation

Operons

• Genes: ypeP-ypeQ

  Description: PubMed

  Regulation: expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-A893 (ypeP::erm), available at the NBRP B. subtilis, Japan
• BP443 (ypeP::tet) available in Fabian Commichau's lab
• BKE21970 (ypeP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACAATCACCTTACCAT,  downstream forward: _UP4_TTAGACCGTAACGGAGATGA
• BKK21970 (ypeP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACAATCACCTTACCAT,  downstream forward: _UP4_TTAGACCGTAACGGAGATGA

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, based on pGP380,  expression from plasmid: pBP501 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

Research papers

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