lepA

168

elongation factor 4, GTP-binding protein

Locus: BSU_25510

Molecular weight: 68.41 kDa

Isoelectric point: 4.98

Protein length: 612 aa

Gene length: 1839 bp

Function: translation elongation

Product: elongation factor 4

Essential: no

Synonyms: lepA, yqxB, yqeQ

Genomic Context

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List of homologs in different organisms, belongs to COG0481 (Galperin et al., 2021)

Gene

Coordinates: 2,630,910  2,632,748

The protein

Catalyzed reaction/ biological activity

• GTP + H₂O --> GDP + H⁺ + phosphate (according to UniProt)

Protein family

• TRAFAC class translation factor GTPase superfamily (according to UniProt)
• Classic translation factor GTPase family (according to UniProt)

Domains

• tr-type G domain (aa 12-194) (according to UniProt)

Structure

• 4QJT (PDB) (LepA bound to the Thermus thermophilus ribosome, 62% identity) PubMed
• P37949 (AlphaFold)

Localization

• cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

• Genes: lepA-hemN

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • There is a terminator between 'lepA' and 'hemN' with only little readthrough

Biological materials

Mutant

• BKE25510 (lepA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATCTATCACTCCTACT,  downstream forward: _UP4_TAGAAGCCGCCGCAGTCTTG
• BKK25510 (lepA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATCTATCACTCCTACT,  downstream forward: _UP4_TAGAAGCCGCCGCAGTCTTG

References

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