spoIIIAH

168

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Locus: BSU_24360

Molecular weight: 23.58 kDa

Isoelectric point: 4.63

Protein length: 218 aa

Gene length: 657 bp

Function: activation of SigG, forespore encasement by the spore coat

Product: part of the transmembrane channel linking the mother cell and the forespore

Essential: no

Synonyms: spoIIIAH

Genomic Context

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List of homologs in different organisms, belongs to COG5829 (Galperin et al., 2021)

Gene

Coordinates: 2,532,353  2,533,009

Phenotypes of a mutant

• reduced sporulation efficiency (1 to 10% compared to wild type) PubMed
• the ''spoIIIL spoIIIAH'' double mutant has a severe sporulation defect (0.001%) PubMed
• block of sporulation after engulfment

The protein

Catalyzed reaction/ biological activity

• required for forespore encasement by the spore coat PubMed

Domains

• contains a ring-building motif (RBM) domain PubMed

Structure

• 3UZO (PDB); 3TUF (PDB) (the SpoIIQ-SpoIIIAH pore forming complex)  PubMed
• P49785 (AlphaFold)

Localization

• membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with SpoIIQ) PubMed
• proper recruitment to the sporulation septum on the mother cell side requires SpoIIQ PubMed

Expression and Regulation

Operons

• Genes: yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Additional information

  • for a discussion of the relevance of the operon-internal promoter, see PubMed

• Genes: spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

Biological materials

Mutant

• BKE24360 (spoIIIAH::erm trpC2)available at BGSC,  PubMed, upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA,  downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC
• BKK24360 (spoIIIAH::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA,  downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

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Original Publications

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