Difference between revisions of "FlhA"

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* '''Description:''' flagella-associated protein <br/><br/>
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* '''Description:''' part of the flagellar type III export apparatus <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || flagella-associated protein
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|style="background:#ABCDEF;" align="center"| '''Product''' || part of the flagellar type III export apparatus
 
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis
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|style="background:#ABCDEF;" align="center"|'''Function''' || export of flagellum components
 
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|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16390 flhA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16390 flhA]
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[motility and chemotaxis]]}},
 
{{SubtiWiki category|[[motility and chemotaxis]]}},
 +
{{SubtiWiki category|[[protein secretion]]}},
 
{{SubtiWiki category|[[membrane proteins]]}}
 
{{SubtiWiki category|[[membrane proteins]]}}
  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* no secretion of [[FlgM]], permanent inhibition of [[SigD]] {{PubMed|25313396}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
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−
 
−
 
  
 
=The protein=
 
=The protein=
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=References=
 
=References=
  
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<pubmed>8097015, 20534509,14651647,9657996,8157612,15175317 17850253</pubmed>
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<pubmed>8097015, 20534509,14651647,9657996,8157612,15175317 17850253 25313396</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:14, 27 October 2014

  • Description: part of the flagellar type III export apparatus

Gene name flhA
Synonyms
Essential no
Product part of the flagellar type III export apparatus
Function export of flagellum components
Gene expression levels in SubtiExpress: flhA
Interactions involving this protein in SubtInteract: FlhA
MW, pI 73 kDa, 4.637
Gene length, protein length 2031 bp, 677 aa
Immediate neighbours flhB, flhF
Sequences Protein DNA DNA_with_flanks
Genetic context
FlhA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FlhA expression.png















Categories containing this gene/protein

motility and chemotaxis, protein secretion, membrane proteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16390

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: FHIPEP (flagella/HR/invasion proteins export pore) family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 167 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Rebecca A Calvo, Daniel B Kearns
FlgM is secreted by the flagellar export apparatus in Bacillus subtilis.
J Bacteriol: 2015, 197(1);81-91
[PubMed:25313396] [WorldCat.org] [DOI] (I p)

Gert Bange, Nico Kümmerer, Christoph Engel, Gunes Bozkurt, Klemens Wild, Irmgard Sinning
FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system.
Proc Natl Acad Sci U S A: 2010, 107(25);11295-300
[PubMed:20534509] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996] [WorldCat.org] [DOI] (P p)

L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612] [WorldCat.org] [DOI] (P p)

P B Carpenter, G W Ordal
Bacillus subtilis FlhA: a flagellar protein related to a new family of signal-transducing receptors.
Mol Microbiol: 1993, 7(5);735-43
[PubMed:8097015] [WorldCat.org] [DOI] (P p)

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