rsbP

168

protein serine phosphatase, energy PP2C, dephosphorylates RsbV, required for activation of SigB during bacterial-fungal interaction

Locus: BSU_34110

Molecular weight: 45.87 kDa

Isoelectric point: 4.83

Protein length: 403 aa

Gene length: 1212 bp

Function: control of SigB activity

Product: protein serine phosphatase, energy PP2C

Essential: no

E.C.: 3.1.3.3

Synonyms: rsbP, yvfP

Genomic Context

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List of homologs in different organisms, belongs to COG2208 (Galperin et al., 2021)

Gene

Coordinates: 3,500,386  3,501,597

Phenotypes of a mutant

• no induction of σ^B-dependent transcripts in response to carbon limitation

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of RsbV in response to red light, this results in SigB activation PubMed
• H₂O + O-phospho-L(or D)-serine --> L(or D)-serine + phosphate (according to UniProt)

Domains

• N-terminal PAS domain, central coiled-coil domain
• PPM-type phosphatase domain (aa 191-402) (according to UniProt)

Structure

• O07014 (AlphaFold)

Effectors of protein activity

• activity is stimulated upon interaction of the RsbP oligomer with RsbQ PubMed

Expression and Regulation

Operons

• Genes: rsbP-rsbQ

  Description: PubMed

Biological materials

Mutant

• GP3323 (ΔrsbP::phleo), available in Jörg Stülke's lab
• MGNA-A488 (yvfP::erm), available at the NBRP B. subtilis, Japan
• BKE34110 (rsbP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAAGAGGGTCACCTCT,  downstream forward: _UP4_TAAAATGATCCATGAGACAT
• BKK34110 (rsbP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAAGAGGGTCACCTCT,  downstream forward: _UP4_TAAAATGATCCATGAGACAT

Expression vectors

• pGP3599: expression of rsbP by pBQ200 in B. subtilis, available in Jörg Stülke's lab

LacZ fusion

• GP3328 (based on pAC5), available in Jörg Stülke's lab

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Reviews

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Original Publications

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