walK

168

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors

Locus: BSU_40400

Molecular weight: 69.86 kDa

Isoelectric point: 4.78

Protein length: 611 aa

Gene length: 1836 bp

Function: control of cell wall metabolism

Product: two-component sensor kinase

Essential: yes

E.C.: 2.7.13.3

Synonyms: walK, yycG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5002 (Galperin et al., 2021)

Gene

Coordinates: 4,151,853 → 4,153,688

Phenotypes of a mutant

• essential PubMed
• essentiality can be bypassed by constitutive expression of lytE or cwlO in a pdaC iseA mutant background PubMed
• a mutant (point mutation) has been selected during growth at low pressure PubMed

Additional information

• the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots PubMed

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of WalR
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Protein family

• two-component sensor kinase

Domains

• two transmembrane segments
• HAMP domain (aa 204-256) (according to UniProt)
• PAS domain (aa 263-324) (according to UniProt)
• PAC domain (aa 325-379) (according to UniProt)
• Histidine kinase domain (aa 383-602) (according to UniProt)

Structure

• 3SL2 (PDB) (complex of the ATP binding histidine kinase domain with ATP) PubMed
• Q45614 (AlphaFold)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• activity is modulated in response to changes in D,L-endopeptidase activity (via the extracellular PAS domain) PubMed

Paralogous protein(s)

• ResE, PhoR

Localization

• cell membrane PubMed
• part of the division septum in growing cells, interacts with the divisome PubMed

Expression and Regulation

Operons

• Genes: walR-walK-walH-walI-walJ-htrC

  Description: PubMed

  Regulation

  • htrC transcript expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B827 (yycG::erm), available at the NBRP B. subtilis, Japan

Expression vectors

• pGP2636, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab

References

Reviews

Loading

Original Publications

Loading