Difference between revisions of "AbrB"

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(Other original publications)
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00370&redirect=T BSU00370]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00370&redirect=T BSU00370]
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Z0R 1Z0R] (N-terminal DNA recognition domain), [http://www.ncbi.nlm.nih.gov/sites/entrez/16223496 PubMed]
+
* '''Structure:'''  
 +
** [http://www.rcsb.org/pdb/explore.do?structureId=1Z0R 1Z0R] (N-terminal DNA recognition domain), [http://www.ncbi.nlm.nih.gov/sites/entrez/16223496 PubMed]
 +
** [http://www.rcsb.org/pdb/explore.do?structureId=2MJG 2MJG] (full-length protein) {{PubMed|25308864}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P08874 P08874]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P08874 P08874]
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<pubmed>20817675,18840696 23580539</pubmed>
 
<pubmed>20817675,18840696 23580539</pubmed>
 
==Other original publications==
 
==Other original publications==
<pubmed> 19581368 ,3145384,2437099,8878039,2504584, 11377867,1850083,2106683,12586407, 12076816,17660417, 2554317,18430133,7768874, 2507867, 1766371, 15687200,1908787,2106683,15687200, 2504584,3145384 8821944 8576231 11101897 11395475 11583849 11751836 12123659 12076816 12591885 15610005 16223496 16159768 16702211 17660417 17720793 7592460, 19465659  20509597 15101989 19202088,18326573 24534728 24731262 24832089 25002359 </pubmed>
+
<pubmed> 19581368 ,3145384,2437099,8878039,2504584, 11377867,1850083,2106683,12586407, 12076816,17660417, 2554317,18430133,7768874, 2507867, 1766371, 15687200,1908787,2106683,15687200, 2504584,3145384 8821944 8576231 11101897 11395475 11583849 11751836 12123659 12076816 12591885 15610005 16223496 16159768 16702211 17660417 17720793 7592460, 19465659  20509597 15101989 19202088,18326573 24534728 24731262 24832089 25002359 25308864 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 07:59, 14 October 2014

  • Description: transcriptional regulator of transition state genes

Gene name abrB
Synonyms cpsX, tolB
Essential no
Product transcriptional regulator
Function regulation of gene expression during the transition
from growth to stationary phase
Gene expression levels in SubtiExpress: abrB
Interactions involving this protein in SubtInteract: AbrB
Metabolic function and regulation of this protein in SubtiPathways:
abrB
MW, pI 10 kDa, 6.57
Gene length, protein length 282 bp, 94 aa
Immediate neighbours yabC, metS
Sequences Protein DNA DNA_with_flanks
Genetic context
AbrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AbrB expression.png















Categories containing this gene/protein

transition state regulators

This gene is a member of the following regulons

AbrB regulon, Spo0A regulon

The AbrB regulon

The gene

Basic information

  • Locus tag: BSU00370

Phenotypes of a mutant

  • No swarming motility on B medium PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): Abh, SpoVT (only N-terminal domain)

Extended information on the protein

  • Kinetic information:
  • Effectors of protein activity: interaction with AbbA results in inactivation of AbrB PubMed

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: expressed at the onset of stationary phase PubMed
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2859 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 10811 PubMed

Biological materials

  • Mutant: TT731 (aphA3)
    • 1A935 (abrB::cat), PubMed, available at BGSC
    • GP1548 (abrB::ermC), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Mark Strauch, Baltimore, USA homepage

Your additional remarks

References

Reviews

The AbrB regulon:

Other original publications

Andrew L Olson, Ashley T Tucker, Benjamin G Bobay, Erik J Soderblom, M Arthur Moseley, Richele J Thompson, John Cavanagh
Structure and DNA-binding traits of the transition state regulator AbrB.
Structure: 2014, 22(11);1650-6
[PubMed:25308864] [WorldCat.org] [DOI] (I p)

Astrid Magdalena Lozano Goné, Jabel Dinorín Téllez Girón, Fabiola Eloisa Jiménez Montejo, María Eugenia Hidalgo-Lara, Víctor Eric López Y López
Behavior of transition state regulator AbrB in batch cultures of Bacillus thuringiensis.
Curr Microbiol: 2014, 69(5);725-32
[PubMed:25002359] [WorldCat.org] [DOI] (I p)

Svetlana Neubauer, Olga Dolgova, Gregory Präg, Rainer Borriss, Oliwia Makarewicz
Substitutional analysis of the C-terminal domain of AbrB revealed its essential role in DNA-binding activity.
PLoS One: 2014, 9(5);e97254
[PubMed:24832089] [WorldCat.org] [DOI] (I e)

Ahasanul Kobir, Sandrine Poncet, Vladimir Bidnenko, Olivier Delumeau, Carsten Jers, Samira Zouhir, Rosa Grenha, Sylvie Nessler, Phillipe Noirot, Ivan Mijakovic
Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties.
Mol Microbiol: 2014, 92(5);1129-41
[PubMed:24731262] [WorldCat.org] [DOI] (I p)

Ashley T Tucker, Benjamin G Bobay, Allison V Banse, Andrew L Olson, Erik J Soderblom, M Arthur Moseley, Richele J Thompson, Kristen M Varney, Richard Losick, John Cavanagh
A DNA mimic: the structure and mechanism of action for the anti-repressor protein AbbA.
J Mol Biol: 2014, 426(9);1911-24
[PubMed:24534728] [WorldCat.org] [DOI] (I p)

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

Steve D Seredick, Barbara M Seredick, David Baker, George B Spiegelman
An A257V mutation in the bacillus subtilis response regulator Spo0A prevents regulated expression of promoters with low-consensus binding sites.
J Bacteriol: 2009, 191(17);5489-98
[PubMed:19581368] [WorldCat.org] [DOI] (I p)

Yun Luo, John D Helmann
Extracytoplasmic function sigma factors with overlapping promoter specificity regulate sublancin production in Bacillus subtilis.
J Bacteriol: 2009, 191(15);4951-8
[PubMed:19465659] [WorldCat.org] [DOI] (I p)

Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088] [WorldCat.org] [DOI] (P p)

Frances Chu, Daniel B Kearns, Anna McLoon, Yunrong Chai, Roberto Kolter, Richard Losick
A novel regulatory protein governing biofilm formation in Bacillus subtilis.
Mol Microbiol: 2008, 68(5);1117-27
[PubMed:18430133] [WorldCat.org] [DOI] (I p)

Krzysztofa Nagórska, Krzysztof Hinc, Mark A Strauch, Michał Obuchowski
Influence of the sigmaB stress factor and yxaB, the gene for a putative exopolysaccharide synthase under sigmaB Control, on biofilm formation.
J Bacteriol: 2008, 190(10);3546-56
[PubMed:18326573] [WorldCat.org] [DOI] (I p)

Mark A Strauch, Benjamin G Bobay, John Cavanagh, Fude Yao, Angelo Wilson, Yoann Le Breton
Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.
J Bacteriol: 2007, 189(21);7720-32
[PubMed:17720793] [WorldCat.org] [DOI] (P p)

Sina Jordan, Eva Rietkötter, Mark A Strauch, Falk Kalamorz, Bronwyn G Butcher, John D Helmann, Thorsten Mascher
LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis.
Microbiology (Reading): 2007, 153(Pt 8);2530-2540
[PubMed:17660417] [WorldCat.org] [DOI] (P p)

Benjamin G Bobay, Geoffrey A Mueller, Richele J Thompson, Alexey G Murzin, Ronald A Venters, Mark A Strauch, John Cavanagh
NMR structure of AbhN and comparison with AbrBN: FIRST insights into the DNA binding promiscuity and specificity of AbrB-like transition state regulator proteins.
J Biol Chem: 2006, 281(30);21399-21409
[PubMed:16702211] [WorldCat.org] [DOI] (P p)

Benjamin G Bobay, Antonina Andreeva, Geoffrey A Mueller, John Cavanagh, Alexey G Murzin
Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins.
FEBS Lett: 2005, 579(25);5669-74
[PubMed:16223496] [WorldCat.org] [DOI] (P p)

Fude Yao, Mark A Strauch
Independent and interchangeable multimerization domains of the AbrB, Abh, and SpoVT global regulatory proteins.
J Bacteriol: 2005, 187(18);6354-62
[PubMed:16159768] [WorldCat.org] [DOI] (P p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Benjamin G Bobay, Linda Benson, Stephen Naylor, Brett Feeney, A Clay Clark, Michael B Goshe, Mark A Strauch, Richele Thompson, John Cavanagh
Evaluation of the DNA binding tendencies of the transition state regulator AbrB.
Biochemistry: 2004, 43(51);16106-18
[PubMed:15610005] [WorldCat.org] [DOI] (P p)

Mélanie A Hamon, Nicola R Stanley, Robert A Britton, Alan D Grossman, Beth A Lazazzera
Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis.
Mol Microbiol: 2004, 52(3);847-60
[PubMed:15101989] [WorldCat.org] [DOI] (P p)

Hyun-Jin Kim, Sam-In Kim, Manoja Ratnayake-Lecamwasam, Kiyoshi Tachikawa, Abraham L Sonenshein, Mark Strauch
Complex regulation of the Bacillus subtilis aconitase gene.
J Bacteriol: 2003, 185(5);1672-80
[PubMed:12591885] [WorldCat.org] [DOI] (P p)

Leendert W Hamoen, Daisy Kausche, Mohamed A Marahiel, Douwe van Sinderen, Gerard Venema, Pascale Serror
The Bacillus subtilis transition state regulator AbrB binds to the -35 promoter region of comK.
FEMS Microbiol Lett: 2003, 218(2);299-304
[PubMed:12586407] [WorldCat.org] [DOI] (P p)

Linda M Benson, Jeffrey L Vaughn, Mark A Strauch, Benjamin G Bobay, Richele Thompson, Stephen Naylor, John Cavanagh
Macromolecular assembly of the transition state regulator AbrB in its unbound and complexed states probed by microelectrospray ionization mass spectrometry.
Anal Biochem: 2002, 306(2);222-7
[PubMed:12123659] [WorldCat.org] [DOI] (P p)

Qiang Qian, Chien Y Lee, John D Helmann, Mark A Strauch
AbrB is a regulator of the sigma(W) regulon in Bacillus subtilis.
FEMS Microbiol Lett: 2002, 211(2);219-23
[PubMed:12076816] [WorldCat.org] [DOI] (P p)

Sasha H Shafikhani, Ines Mandic-Mulec, Mark A Strauch, Issar Smith, Terrance Leighton
Postexponential regulation of sin operon expression in Bacillus subtilis.
J Bacteriol: 2002, 184(2);564-71
[PubMed:11751836] [WorldCat.org] [DOI] (P p)

Z E Phillips, M A Strauch
Role of Cys54 in AbrB multimerization and DNA-binding activity.
FEMS Microbiol Lett: 2001, 203(2);207-10
[PubMed:11583849] [WorldCat.org] [DOI] (P p)

K Xu, M A Strauch
DNA-binding activity of amino-terminal domains of the Bacillus subtilis AbrB protein.
J Bacteriol: 2001, 183(13);4094-8
[PubMed:11395475] [WorldCat.org] [DOI] (P p)

M C Gustafsson, C von Wachenfeldt
A novel diffusible substance can overcome the apparent AbrB repression of the Bacillus subtilis fatR promoter.
FEMS Microbiol Lett: 2001, 199(2);197-202
[PubMed:11377867] [WorldCat.org] [DOI] (P p)

J L Vaughn, V Feher, S Naylor, M A Strauch, J Cavanagh
Novel DNA binding domain and genetic regulation model of Bacillus subtilis transition state regulator abrB.
Nat Struct Biol: 2000, 7(12);1139-46
[PubMed:11101897] [WorldCat.org] [DOI] (P p)

J Hahn, A Luttinger, D Dubnau
Regulatory inputs for the synthesis of ComK, the competence transcription factor of Bacillus subtilis.
Mol Microbiol: 1996, 21(4);763-75
[PubMed:8878039] [WorldCat.org] [DOI] (P p)

K Xu, D Clark, M A Strauch
Analysis of abrB mutations, mutant proteins, and why abrB does not utilize a perfect consensus in the -35 region of its sigma A promoter.
J Biol Chem: 1996, 271(5);2621-6
[PubMed:8576231] [WorldCat.org] [DOI] (P p)

K Xu, M A Strauch
In vitro selection of optimal AbrB-binding sites: comparison to known in vivo sites indicates flexibility in AbrB binding and recognition of three-dimensional DNA structures.
Mol Microbiol: 1996, 19(1);145-58
[PubMed:8821944] [WorldCat.org] [DOI] (P p)

M A Strauch
AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon.
J Bacteriol: 1995, 177(23);6727-31
[PubMed:7592460] [WorldCat.org] [DOI] (P p)

J Hahn, M Roggiani, D Dubnau
The major role of Spo0A in genetic competence is to downregulate abrB, an essential competence gene.
J Bacteriol: 1995, 177(12);3601-5
[PubMed:7768874] [WorldCat.org] [DOI] (P p)

R Fürbass, M A Marahiel
Mutant analysis of interaction of the Bacillus subtilis transcription regulator AbrB with the antibiotic biosynthesis gene tycA.
FEBS Lett: 1991, 287(1-2);153-6
[PubMed:1908787] [WorldCat.org] [DOI] (P p)

F J Slack, J P Mueller, M A Strauch, C Mathiopoulos, A L Sonenshein
Transcriptional regulation of a Bacillus subtilis dipeptide transport operon.
Mol Microbiol: 1991, 5(8);1915-25
[PubMed:1766371] [WorldCat.org] [DOI] (P p)

R Fürbass, M Gocht, P Zuber, M A Marahiel
Interaction of AbrB, a transcriptional regulator from Bacillus subtilis with the promoters of the transition state-activated genes tycA and spoVG.
Mol Gen Genet: 1991, 225(3);347-54
[PubMed:1850083] [WorldCat.org] [DOI] (P p)

M Strauch, V Webb, G Spiegelman, J A Hoch
The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene.
Proc Natl Acad Sci U S A: 1990, 87(5);1801-5
[PubMed:2106683] [WorldCat.org] [DOI] (P p)

J B Robertson, M Gocht, M A Marahiel, P Zuber
AbrB, a regulator of gene expression in Bacillus, interacts with the transcription initiation regions of a sporulation gene and an antibiotic biosynthesis gene.
Proc Natl Acad Sci U S A: 1989, 86(21);8457-61
[PubMed:2554317] [WorldCat.org] [DOI] (P p)

M A Strauch, M Perego, D Burbulys, J A Hoch
The transition state transcription regulator AbrB of Bacillus subtilis is autoregulated during vegetative growth.
Mol Microbiol: 1989, 3(9);1203-9
[PubMed:2507867] [WorldCat.org] [DOI] (P p)

M A Strauch, G B Spiegelman, M Perego, W C Johnson, D Burbulys, J A Hoch
The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
EMBO J: 1989, 8(5);1615-21
[PubMed:2504584] [WorldCat.org] [DOI] (P p)

M Perego, G B Spiegelman, J A Hoch
Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis.
Mol Microbiol: 1988, 2(6);689-99
[PubMed:3145384] [WorldCat.org] [DOI] (P p)

P Zuber, R Losick
Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis.
J Bacteriol: 1987, 169(5);2223-30
[PubMed:2437099] [WorldCat.org] [DOI] (P p)