Difference between revisions of "YpeP"

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(Biological materials)
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* '''Mutant:''' BP443 (''ΔypeP::tet'') available in [[Fabian Commichau]]'s lab
 
* '''Mutant:''' BP443 (''ΔypeP::tet'') available in [[Fabian Commichau]]'s lab
  
* '''Expression vector:'''
+
* '''Expression vector:''' for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from plasmid: pBP501 (E. coli amp & B. subtilis E/L) , available in [[Fabian Commichau]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
Line 126: Line 126:
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Fabian Commichau]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 13:28, 7 October 2014

  • Description: unknown

Gene name ypeP
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ypeP
MW, pI 25.85 kDa, 5.235
Gene length, protein length 681 bp, 226 aa
Immediate neighbours ypeQ, ypdP
Sequences Protein DNA DNA_with_flanks
Genetic context
YpeP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YpeP expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU21970

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector: for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from plasmid: pBP501 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References