rsiV

168

anti-sigma factor to SigV

Locus: BSU_27130

Molecular weight: 32.72 kDa

Isoelectric point: 5.83

Protein length: 285 aa

Gene length: 858 bp

Function: control of SigV activity

Product: anti-sigma factor to SigV

Essential: no

Synonyms: rsiV, yrhM

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,770,350 → 2,771,207

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed

The protein

Catalyzed reaction/ biological activity

• binds and thus inactivates SigV

Structure

• 5JEN (PDB) (complex with lysozyme)
• O05403 (AlphaFold)

Effectors of protein activity

• RsiV directly binds lysozyme  PubMed
• upon exposure to lysozyme, the extracellular domain of RsiV is cleaved off by SipS and SipT (between A66 and M67), subsequently the N-terminal part of the protein is subject to intramemrane proteolysis by RasP PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: sigV-rsiV-oatA-yrhK

  Description: PubMed

  Regulation

  • induced by lysozyme (SigV) PubMed

  Sigma factors

  • SigV: sigma factor, PubMed, in sigV regulon

  Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed

Biological materials

Mutant

• MGNA-A145 (yrhM::erm), available at the NBRP B. subtilis, Japan
• BKE27130 (ΔrsiV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAATTGCTGTAATCTCTTAT,  downstream forward: _UP4_TAAAATAAAAACATCCATCT
• BKK27130 (ΔrsiV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAATTGCTGTAATCTCTTAT,  downstream forward: _UP4_TAAAATAAAAACATCCATCT

References

Reviews

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Original Publications

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