fliW

168

checkpoint protein for hag expression, CsrA antagonist

Locus: BSU_35380

Molecular weight: 16.03 kDa

Isoelectric point: 4.34

Protein length: 143 aa

Gene length: 432 bp

Function: control of CsrA activity

Product: flagellar assembly factor, CsrA antagonist

Essential: no

Synonyms: fliW, yviF

Genomic Context

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List of homologs in different organisms, belongs to COG1699 (Galperin et al., 2021)

Gene

Coordinates: 3,636,264  3,636,695

The protein

Catalyzed reaction/ biological activity

• FliW binds CsrA when Hag is secreted after hook completion (and FliW released from the Hag-FliW complex) to allow translation of the'' hag'' mRNA PubMed

Protein family

• fliW family (single member, according to UniProt)

Structure

• 5DMD (PDB) (from Geobacillus thermodenitrificans) PubMed
• 5DMB (PDB) (FliW-CsrA complex) PubMed
• P96503 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• CsrA and FliW form a conserved module in many bacteria PubMed

Expression and Regulation

Operons

• Genes: comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA

  Description: PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon
  • DegU: activation, DegU-P, PubMed, in degU regulon
  • ScoC: repression, PubMed, in scoC regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• MGNA-A339 (yviF::erm), available at the NBRP B. subtilis, Japan
• BKE35380 (fliW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTCACGATCCTTTTC,  downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
• BKK35380 (fliW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTCACGATCCTTTTC,  downstream forward: _UP4_AAGCATCCGATTGGAGGAGA

References

Reviews

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Original Publications

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