Difference between revisions of "SpsA"

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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' glycosyltransferase 2 family (according to Swiss-Prot)
+
* '''Protein family:''' glycosyltransferase 2 family {{PubMed|10350455}}
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''

Revision as of 10:49, 23 September 2014

  • Description: nucleotide-sugar-dependent glycosyltransferase, spore coat polysaccharide synthesis

Gene name spsA
Synonyms ipa-63d
Essential no
Product nucleotide-sugar-dependent glycosyltransferase
Function spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsA
MW, pI 30 kDa, 5.358
Gene length, protein length 768 bp, 256 aa
Immediate neighbours spsB, gerQ
Sequences Protein DNA DNA_with_flanks
Genetic context
SpsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpsA expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU37910

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: glycosyltransferase 2 family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

N Tarbouriech, S J Charnock, G J Davies
Three-dimensional structures of the Mn and Mg dTDP complexes of the family GT-2 glycosyltransferase SpsA: a comparison with related NDP-sugar glycosyltransferases.
J Mol Biol: 2001, 314(4);655-61
[PubMed:11733986] [WorldCat.org] [DOI] (P p)

S J Charnock, G J Davies
Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms.
Biochemistry: 1999, 38(20);6380-5
[PubMed:10350455] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)