ycnK

168

copper-responsive transcription repressor of the ycnK-ycnJ-ycnI operon, DeoR family

Locus: BSU_03960

Molecular weight: 21.29 kDa

Isoelectric point: 7.37

Protein length: 190 aa

Gene length: 573 bp

Function: regulation of copper uptake

Product: transcription repressor, DeoR family

Essential: no

Synonyms: ycnK

Genomic Context

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List of homologs in different organisms, belongs to COG1349 (Galperin et al., 2021)

Gene

Coordinates: 448,461  449,033

Phenotypes of a mutant

• elevated expression of ''ycnJ'' under conditions of copper excess PubMed

The protein

Catalyzed reaction/ biological activity

• repression of ''ycnK-ycnJ-ycnI'' expression in the presence of excess copper PubMed

Protein family

• DeoR family of transcription factors

Domains

• HTH deoR-type domain (aa 3-58) (according to UniProt)

Cofactors

• copper acts as corepressor PubMed

Structure

• P94433 (AlphaFold)

Expression and Regulation

Operons

• Genes: ycnK-ycnJ-ycnI

  Description: PubMed

  Regulation

  • induced by copper limitation (YcnK) PubMed

  Regulatory mechanism

  • YcnK: repression, PubMed, in ycnK regulon
  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C017 (ycnK::erm), available at the NBRP B. subtilis, Japan
• BKE03960 (ycnK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATACACCCTCTTCAT,  downstream forward: _UP4_TAACACAACACATACAGCGG
• BKK03960 (ycnK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATACACCCTCTTCAT,  downstream forward: _UP4_TAACACAACACATACAGCGG

Labs working on this gene/protein

• Mohamed Marahiel, Marburg University, Germany homepage

References

Reviews

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Original Publications

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