htrA

168

membrane-anchored protein quality control protease, serine protease Do

Locus: BSU_12900

Molecular weight: 47.55 kDa

Isoelectric point: 4.7

Protein length: 449 aa

Gene length: 1350 bp

Function: protein quality control

Product: serine protease Do

Essential: no

Synonyms: htrA, ykdA

Genomic Context

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List of homologs in different organisms, belongs to COG0265 (Galperin et al., 2021)

Gene

Coordinates: 1,357,936  1,359,285

The protein

Catalyzed reaction/ biological activity

• required for folding of the secreted protein YqxI PubMed
• Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val (according to UniProt)

Protein family

• Peptidase S1C family (with HtrB and HtrC, according to UniProt)

Domains

• cytoplasmic domain (aa 1 - 44) (according to UniProt)
• transmembrane helix (aa 45 - 67) (according to UniProt)
• extracellular domain (aa 68 - 479) (according to UniProt)
• PDZ domain (aa 342 - 441) (according to UniProt)

Structure

• 3QO6 (PDB) (from Arabidopsis thaliana, 37% identity) PubMed
• O34358 (AlphaFold)

Paralogous protein(s)

• HtrC

Localization

• cell membrane (according to Swiss-Prot)
• extracellular (signal peptide) PubMed
• randomly distributed in foci throughout the cell surface PubMed

Expression and Regulation

Operons

• Genes: htrA

  Description: PubMed

  Regulation

  • expressed under conditions of secretion stress (CssR) PubMed

  Regulatory mechanism

  • CssR: activation, PubMed, in cssR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • HtrA is subject to degradation by WprA and other extracellular proteases PubMed

Biological materials

Mutant

• 1A933 (no resistance), PubMed, available at BGSC
• BKE12900 (htrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATGTTCACTCCGTTTC,  downstream forward: _UP4_TAAGACATAATGCCTCAGGC
• BKK12900 (htrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATGTTCACTCCGTTTC,  downstream forward: _UP4_TAAGACATAATGCCTCAGGC

References

Reviews

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Original Publications

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