cadA

168

cadmium transporting ATPase, resistance to cadmium

Locus: BSU_33490

Molecular weight: 75.22 kDa

Isoelectric point: 6

Protein length: 699 aa

Gene length: 2100 bp

Function: cadmium export

Product: cadmium transporting ATPase

Essential: no

E.C.: 3.6.3.3

Synonyms: cadA, yvgW

Genomic Context

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List of homologs in different organisms, belongs to COG2217 (Galperin et al., 2021)

Gene

Coordinates: 3,438,853  3,440,952

The protein

Catalyzed reaction/ biological activity

• ATP + Cd²⁺ + H₂O --> ADP + Cd²⁺ + H⁺ + phosphate (according to UniProt)
• ATP + H₂O + Zn²⁺ --> ADP + H⁺ + phosphate + Zn²⁺ (according to UniProt)

Protein family

• cation transport ATPase (P-type) (TC 3.A.3) family (according to UniProt)

Domains

• HMA domain (aa 5-73) (according to UniProt)

Structure

• 4BBJ (PDB) (CopA from Legionella pneumophila, 32% identity, 67% similarity) PubMed
• O32219 (AlphaFold)

Paralogous protein(s)

• CopA, PfeT

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cadA

  Description: PubMed

  Regulation

  • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed

  Regulatory mechanism

  • CzrA: repression, PubMed, in czrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A491 (yvgW::erm), available at the NBRP B. subtilis, Japan
• BKE33490 (cadA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTAGTCTCACCTTACCTT,  downstream forward: _UP4_TAAATTGTCGGAGAGAATTC
• BKK33490 (cadA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTAGTCTCACCTTACCTT,  downstream forward: _UP4_TAAATTGTCGGAGAGAATTC

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

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