divIB

168

cell-division initiation protein (septum formation)

Locus: BSU_15240

Molecular weight: 30.02 kDa

Isoelectric point: 9.24

Protein length: 263 aa

Gene length: 792 bp

Function: septum formation

Product: cell-division protein, member of the divisome

Essential: yes

Synonyms: divIB, dds, tms-12

Genomic Context

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List of homologs in different organisms, belongs to COG1589 (Galperin et al., 2021)

Gene

Coordinates: 1,593,704 → 1,594,495

Phenotypes of a mutant

• essential PubMed

The protein

Protein family

• FtsQ/DivIB family (single member, according to UniProt)

Domains

• POTRA domain (aa 54-123) (according to UniProt)

Structure

• 2ALJ (PDB) (major extracytoplasmic domain, Geobacillus stearothermophilus)
• P16655 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigM: sigma factor, (promoter within MurG) PubMed, in sigM regulon
  • SigA: sigma factor, PubMed, in sigA regulon
  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoVE-murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

• Genes: divIB-ylxW-ylxX-sbp

  Description: PubMed

• Genes: murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

Biological materials

FLAG-tag construct

• GP2005 divIB-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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