mstX

168

membrane-integrating protein for membrane protein expression, MISTIC

Locus: BSU_31321

Molecular weight: 13.00 kDa

Isoelectric point: 4

Protein length: 110 aa

Gene length: 333 bp

Function: unknown

Product: MISTIC

Essential: no

Synonyms: mstX

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,218,525 → 3,218,857

Phenotypes of a mutant

• reduced biofilm formation (can be suppressed by inactivation of ''sinR'') PubMed
• overexpression result in increased biofilm formation even in a domesticated strain background PubMed

The protein

Structure

• 1YGM (PDB) PubMed
• Q5BU39 (AlphaFold)

Localization

• integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery PubMed

Expression and Regulation

Operons

• Genes: mstX-yugO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • SinR: repression, PubMed, in sinR regulon

  Additional information

  • potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain PubMed

Biological materials

Mutant

• BKE31321 (ΔmstX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAACCGGATTTCTC,  downstream forward: _UP4_GTATCTGAAGAAGGAGAAAA
• BKK31321 (ΔmstX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAACCGGATTTCTC,  downstream forward: _UP4_GTATCTGAAGAAGGAGAAAA

Reviews

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Original Publications

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