yfnI

168

polyglycerolphosphate lipoteichoic acid synthase, general stress protein, major secreted protein, required for survival at low temperature (4°C)

Locus: BSU_07260

Molecular weight: 73.14 kDa

Isoelectric point: 5.95

Protein length: 639 aa

Gene length: 1920 bp

Function: biosynthesis of lipoteichoic acid

Product: minor lipoteichoic acid synthetase

Essential: no

Synonyms: yfnI, yetP, ltaSA

Genomic Context

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List of homologs in different organisms, belongs to COG1368 (Galperin et al., 2021)

Gene

Coordinates: 796,314 → 798,233

Phenotypes of a mutant

• induction of SigM and SigX activities PubMed
• more sensitive to nisin PubMed
• increased conjugation of ICEBs1 PubMed
• suppression of the growth and morphology defects of a glmR mutant PubMed
• inactivation suppresses the essentiality of mbl PubMed

The protein

Protein family

• LTA synthase family (according to UniProt)

Structure

• 2W5Q (PDB) (LtaS from S. aureus, corresponds to aa 215 ... 632 of YfnI) PubMed
• Q797B3 (AlphaFold)

Modification

• phosphorylation on (Thr-311 OR Ser-312) PubMed
• can be phosphorylated by PrkC on Thr-297 in vitro PubMed

Paralogous protein(s)

• YqgS, LtaS, YvgJ

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: yfnI

  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of yfnI PubMed

Biological materials

Mutant

• MGNA-C226 (yfnI::erm), available at the NBRP B. subtilis, Japan
• SM-NB (yfnI-spc), available in Anne Galinier's and Boris Görke's labs
• GP1390 yfnI::spc, available in Jörg Stülke's lab
• GP1397 yfnI::ermC, available in Jörg Stülke's lab
• BKE07260 (ΔyfnI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCAACCTCTACTTTCT,  downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
• BKK07260 (ΔyfnI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCAACCTCTACTTTCT,  downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG

References

Reviews

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Original Publications

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