yhaR

168

similar to enoyl CoA hydratase

Locus: BSU_09880

Molecular weight: 27.47 kDa

Isoelectric point: 6.36

Protein length: 255 aa

Gene length: 768 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhaR

Genomic Context

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Categories containing this gene/protein

Groups of genes, Membrane proteins

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG1024 (Galperin et al., 2021)

This gene is a member of the following regulons

CcpA regulon

Gene

Coordinates: 1,061,491  1,062,258

The protein

Protein family

enoyl-CoA hydratase/isomerase family (according to UniProt)

Structure

3P5M (PDB) (from Mycobacterium avium, 33% identity) PubMed
O07533 (AlphaFold)

Paralogous protein(s)

FadB, (34%)
YngF, (34%)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: yhaR

Description: PubMed

Regulation

expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Regulatory mechanism

CcpA: repression, in ccpA regulon

Biological materials

Mutant

MGNA-B498 (yhaR::erm), available at the NBRP B. subtilis, Japan
BKE09880 (yhaR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAAGCACCTCCATGT,  downstream forward: _UP4_TAACAGATCAGCCGGAAAGC
BKK09880 (yhaR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAAGCACCTCCATGT,  downstream forward: _UP4_TAACAGATCAGCCGGAAAGC

References

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