gcaD

168

bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Locus: BSU_00500

Molecular weight: 49.29 kDa

Isoelectric point: 5.65

Protein length: 456 aa

Gene length: 1371 bp

Function: cell wall metabolism

Product: bifunctional N-acetylglucosamine-1-phosphate

Essential: yes

E.C.: 2.7.7.23

Synonyms: gcaD, tms, tms26, glmU

Genomic Context

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List of homologs in different organisms, belongs to COG1207 (Galperin et al., 2021)

Gene

Coordinates: 56,352  57,722

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + α-D-glucosamine 1-phosphate --> CoA + H⁺ + N-acetyl-α-D-glucosamine 1-phosphate (according to UniProt)
• H⁺ + N-acetyl-α-D-glucosamine 1-phosphate + UTP --> diphosphate + UDP-N-acetyl-α-D-glucosamine (according to UniProt)

Protein family

• N-terminal domain: N-acetylglucosamine-1-phosphate uridyltransferase family (single member, according to UniProt)
• C-terminal domain: Transferase hexapeptide repeat family (according to UniProt)

Structure

• 1HM9 (PDB) (from Streptococcus pneumoniae, 49% identity) PubMed
• P14192 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: gcaD-prs-ctc

  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Expression vectors

• pGP2596: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

References

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