ypgQ

168

Mn²⁺-dependent pyrophosphohydrolase, hydrolyzes (deoxy)ribonucleoside triphosphate [(d)NTP] to (deoxy)ribonucleoside monophosphate and pyrophosphate

Locus: BSU_21890

Molecular weight: 22.98 kDa

Isoelectric point: 5.51

Protein length: 205 aa

Gene length: 618 bp

Function: degradation of excessive or abnormal nucleotides

Product: Mn²⁺-dependent pyrophosphohydrolase

Essential: no

Synonyms: ypgQ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1418 (Galperin et al., 2021)

Gene

Coordinates: 2,303,920  2,304,537

The protein

Domains

• HD domain (aa 22-138) PubMed

Cofactors

• Mn²⁺ PubMed

Structure

• 5IHY (PDB) PubMed
• 5DQV (PDB) PubMed
• 5DQW (PDB) (complexed with ADP) PubMed
• P54168 (AlphaFold)

Expression and Regulation

Operons

• Genes: bsaA-ypgQ-ypgR

  Description: PubMed

Biological materials

Mutant

• MGNA-A888 (ypgQ::erm), available at the NBRP B. subtilis, Japan
• BKE21890 (ypgQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCTTCACAAAAACACCCT,  downstream forward: _UP4_TAATAGAAGGCTTGGAGCGA
• BKK21890 (ypgQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCTTCACAAAAACACCCT,  downstream forward: _UP4_TAATAGAAGGCTTGGAGCGA

References

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