pksL

168

polyketide synthase of type I

Locus: BSU_17190

Molecular weight: 505.85 kDa

Isoelectric point: 5.2

Protein length: 4538 aa

Gene length: 13617 bp

Function: polyketide synthesis

Product: polyketide synthase of type I

Essential: no

Synonyms: pksL, pksX, pksA, outG

Genomic Context

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List of homologs in different organisms, belongs to COG3321 (Galperin et al., 2021)

Gene

Coordinates: 1,807,921 → 1,821,537

The protein

Domains

• 5 Carrier domains (aa 320-394, aa 1800-1873, aa 2597-2674, aa aa 2738-2815, aa 3960-4037) (according to UniProt)

Structure

• 5ENY (PDB) PubMed

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed
  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • CodY: activation, PubMed, in codY regulon

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

Biological materials

Mutant

• BKE17190 (ΔpksL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTTAGACCTCCACCTCATA,  downstream forward: _UP4_TTCAAATAAGAGAGGAGTGG
• BKK17190 (ΔpksL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTTAGACCTCCACCTCATA,  downstream forward: _UP4_TTCAAATAAGAGAGGAGTGG

References

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