katA

168

main vegetative catalase 1

Locus: BSU_08820

Molecular weight: 54.57 kDa

Isoelectric point: 6.15

Protein length: 483 aa

Gene length: 1452 bp

Function: detoxification (degradation) of hydrogen peroxide

Product: vegetative catalase

Essential: no

E.C.: 1.11.1.6

Synonyms: katA, kat-19

Genomic Context

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Categories containing this gene/protein

Lifestyles, Coping with stress, Resistance against oxidative and electrophile stress

Groups of genes, Phosphoproteins, Phosphorylation on an Arg residue

List of homologs in different organisms, belongs to COG0753 (Galperin et al., 2021)

This gene is a member of the following regulons

PerR regulon

Gene

Coordinates: 959,535 → 960,986

Phenotypes of a mutant

increased sensitivity to oxidative stress PubMed
increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed

The protein

Catalyzed reaction/ biological activity

2 H₂O₂ --> 2 H₂O + O₂ (according to UniProt)

Protein family

catalase family (according to UniProt)

Cofactors

contains an iron-sulfur cluster
heme PubMed

Structure

1SI8 (PDB) (enzyme from Enterococcus faecalis, 68% identity)
P26901 (AlphaFold)

Modification

phosphorylated on Arg-366 PubMed

Effectors of protein activity

catalase activity is enhanced upon interaction with YjoB PubMed

Paralogous protein(s)

KatX, KatE

Localization

cytoplasm (according to Swiss-Prot)

Additional information

aggregates at elevated temperatures, aggregation is reduced by interaction with YjoB PubMed

Expression and Regulation

Operons

Genes: katA

Description: PubMed

Regulation

induced in the presence of hydrogen peroxide and paraquat (PerR) PubMed

Regulatory mechanism

PerR: repression, PubMed, in perR regulon

Biological materials

Mutant

GP1726 katA::xeo trpC2 available at Jörg Stülkes lab
GP1725 katA::cat trpC2 available at Jörg Stülkes lab
BKE08820 (ΔkatA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATCACCTCTTGGAA,  downstream forward: _UP4_TAATGGAGAAATGCAAAAAC
BKK08820 (ΔkatA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATCACCTCTTGGAA,  downstream forward: _UP4_TAATGGAGAAATGCAAAAAC

References

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