Difference between revisions of "NonA"

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(Created page with "* '''Description:''' <br/><br/> {| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name''' |''nonA'' |- |style="background:#A...")
 
 
(One intermediate revision by the same user not shown)
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−
* '''Description:''' <br/><br/>
+
* '''Description:''' confers resistance to infection with phage SP10<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || inhibitor of NrdEF of phage SP10
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protection against phage SP10
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 6.094 
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=2879 nonA]
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1795 bp, 329 aa  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8,465 kDa, 9.64 
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 219 bp, 72 aa  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yosP]]'', ''[[nrdEB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yosP]]'', ''[[nrdEB]]''
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
−
{{SubtiWiki category|[[SP-beta prophage]]}}
+
{{SubtiWiki category|[[SP-beta prophage]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}}
 +
 
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
Line 44: Line 48:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* overexpression interferes with respiration (likely due to inhibition of [[NrdE]]) {{PubMed|24272782}}
  
 
=== Database entries ===
 
=== Database entries ===
−
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU20040&redirect=T BSU20040]
+
* '''BsubCyc:''' no entry
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13725]
+
* '''SubtiList entry:''' no entry
  
 
=== Additional information===
 
=== Additional information===
−
 
−
 
−
 
  
 
=The protein=
 
=The protein=
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* '''Protein family:'''
 
* '''Protein family:'''
  
−
* '''Paralogous protein(s):''' [[NrdF]]
+
* '''Paralogous protein(s):'''  
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 +
** membrane {{PubMed|24272782}}
  
 
=== Database entries ===
 
=== Database entries ===
−
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU20040&redirect=T BSU20040]
+
* '''BsubCyc:''' no entry
  
 
* '''Structure:'''
 
* '''Structure:'''
  
−
* '''UniProt:''' [http://www.uniprot.org/uniprot/O30601 O30601]
+
* '''UniProt:''' no entry
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU20040]
+
* '''KEGG entry:''' no entry
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''[[nonA]]'' {{PubMed|24272782}}
  
−
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yosP_2159981_2161778_-1 yosP] {{PubMed|22383849}}
+
* '''Expression browser:''' [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=2879 nonA] {{PubMed|22383849}}
  
−
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' Sig(ORF199-200) of phage SP10 {{PubMed|24272782}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expressed at late stage of phage SP10 infection {{PubMed|24272782}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
 
* '''Additional information:'''
 
* '''Additional information:'''
−
** number of protein molecules per cell (complex medium with amino acids, without glucose): 209 {{PubMed|24696501}}
 
−
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1955 {{PubMed|21395229}}
 
−
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 982 {{PubMed|21395229}}
 
−
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 668 {{PubMed|21395229}}
 
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
−
<pubmed> 11470879 16621400 9465078</pubmed>
+
<pubmed> 24272782 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Latest revision as of 12:49, 22 June 2014

  • Description: confers resistance to infection with phage SP10

Gene name nonA
Synonyms
Essential no
Product inhibitor of NrdEF of phage SP10
Function protection against phage SP10
Gene expression levels in SubtiExpress: nonA
MW, pI 8,465 kDa, 9.64
Gene length, protein length 219 bp, 72 aa
Immediate neighbours yosP, nrdEB
Genetic context
YosP context.gif
This image was kindly provided by SubtiList















Categories containing this gene/protein

SP-beta prophage, membrane proteins


This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: none

Phenotypes of a mutant

  • overexpression interferes with respiration (likely due to inhibition of NrdE) PubMed

Database entries

  • BsubCyc: no entry
  • DBTBS entry: no entry
  • SubtiList entry: no entry

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • BsubCyc: no entry
  • Structure:
  • UniProt: no entry
  • KEGG entry: no entry
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at late stage of phage SP10 infection PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Tatsuya Yamamoto, Nozomu Obana, Lii Mien Yee, Kei Asai, Nobuhiko Nomura, Kouji Nakamura
SP10 infectivity is aborted after bacteriophage SP10 infection induces nonA transcription on the prophage SPβ region of the Bacillus subtilis genome.
J Bacteriol: 2014, 196(3);693-706
[PubMed:24272782] [WorldCat.org] [DOI] (I p)

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