yosP

168

similar to ribonucleoside-diphosphate reductase (beta subunit)

Locus: BSU_20040

Molecular weight: 22.51 kDa

Isoelectric point: 6.09

Protein length: 598 aa

Gene length: 1798 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yosP, nrdF

Genomic Context

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Categories containing this gene/protein

Prophages and mobile genetic elements, Prophages, SP-beta prophage

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG0208 (Galperin et al., 2021)

Gene

Coordinates: 2,159,981  2,161,778

The protein

Catalyzed reaction/ biological activity

[thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H₂O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)

Protein family

ribonucleoside diphosphate reductase small chain family (with NrdF, according to UniProt)

Structure

4DR0 (PDB) ( from Bacillus Subtilis 87% identity)
O30601 (AlphaFold)

Paralogous protein(s)

NrdF

Biological materials

Mutant

BKE20040 (yosP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCCTACATATACGCCAT,  downstream forward: _UP4_TTTACCGAGAAAGGATGTAT
BKK20040 (yosP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCCTACATATACGCCAT,  downstream forward: _UP4_TTTACCGAGAAAGGATGTAT

References

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