dgkB

168

diacylglycerol kinase

Locus: BSU_06720

Molecular weight: 33.18 kDa

Isoelectric point: 5.64

Protein length: 303 aa

Gene length: 912 bp

Function: biosynthesis of lipoteichoic acid

Product: diacylglycerol kinase

Essential: no

E.C.: 2.7.1.107

Synonyms: dgkB, yerQ, dagK

Genomic Context

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List of homologs in different organisms, belongs to COG1597 (Galperin et al., 2021)

Gene

Coordinates: 736,436  737,347

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• essential PubMed, non-essential according to PubMed

The protein

Catalyzed reaction/ biological activity

• 1,2-diacyl-sn-glycerol + ATP --> 1,2-diacyl-sn-glycero-3-phosphate + ADP + H⁺ (according to UniProt)

Protein family

• diacylglycerol/lipid kinase family (with BmrU and YtlR, according to UniProt)

Domains

• DAGKc domain (aa 1-132) (according to UniProt)

Structure

• 3S40 (PDB) (from B. anthracis, 32% identity, 68% similarity)
• O31502 (AlphaFold)

Paralogous protein(s)

• BmrU

Expression and Regulation

Operons

• Genes: dgkB-rlmCD

  Description: PubMed

• Genes: dgkB-rlmCD-yefC-dnmA-yeeB-yeeC

  Description: PubMed

Biological materials

Mutant

• MGNA-B448 (yerQ::erm), available at the NBRP B. subtilis, Japan
• GP1391 dgkB::cat ltaS::aphA3, available in Jörg Stülke's lab
• BKE06720 (dgkB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATCTCATCATCCTACT,  downstream forward: _UP4_TAAAACTTGGCTTGGTAAGC
• BKK06720 (dgkB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATCTCATCATCCTACT,  downstream forward: _UP4_TAAAACTTGGCTTGGTAAGC

References

Reviews

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Original Publications

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