resD

168

two-component response regulator (OmpR family), regulation of aerobic and anaerobic respiration, activates expression of target genes in response to oxygen limitation

Locus: BSU_23120

Molecular weight: 27.34 kDa

Isoelectric point: 5.63

Protein length: 240 aa

Gene length: 723 bp

Function: regulation of aerobic and anaerobic respiration

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: resD, ypxD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 2,417,181  2,417,903

Phenotypes of a mutant

• a resD point mutation has been isolated upon selction at low pressure PubMed

The protein

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 8-121) (according to UniProt)

Structure

• 2OQR (PDB) (RegX3 from Mycobacterium tuberculosis, 37% identity) PubMed
• P35163 (AlphaFold)

Modification

• phosphorylated by ResE on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rluB-resA-resB-resC-resD-resE

  Description: PubMed

• Genes: resA-resB-resC-resD-resE

  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in resD regulon
  • PhoP: activation, PubMed, in phoP regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: resD-resE

  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE23120 (resD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCCCCCTGCTTTC,  downstream forward: _UP4_TATAAATTTGAGGTCGGCGC
• BKK23120 (resD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCCCCCTGCTTTC,  downstream forward: _UP4_TATAAATTTGAGGTCGGCGC

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Reviews

Loading

Original Publications

Loading