sigY

168

RNA polymerase ECF-type sigma factor SigY

Locus: BSU_38700

Molecular weight: 21.21 kDa

Isoelectric point: 9.55

Protein length: 178 aa

Gene length: 537 bp

Function: maintenance of the SP prophage

Product: RNA polymerase ECF-type sigma factor SigY

Essential: no

Synonyms: sigY, yxlB

Genomic Context

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List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

Gene

Coordinates: 3,970,312  3,970,848

Phenotypes of a mutant

• loss of the [[SP prophage]] PubMed
• sensitive to killing by Sublancin]] PubMed
• no production of  Sublancin]] PubMed

The protein

Protein family

• Sigma-70 factor family (according to UniProt)
• ECF subfamily (according to UniProt)

Structure

• 5WUQ (PDB) (the SigW-RsiW complex, 30% identity) PubMed
• P94370 (AlphaFold)

Expression and Regulation

Operons

• Genes: sigY-yxlC-yxlD-yxlE-yxlF-yxlG

  Description: PubMed

  Sigma factors

  • SigY: sigma factor, PubMed, in sigY regulon

Biological materials

Mutant

• MGNA-B751 (sigY::erm), available at the NBRP B. subtilis, Japan
• 1A909 ( sigY::erm), PubMed, available at BGSC
• BKE38700 (sigY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGACCGTGATCCCCCCT,  downstream forward: _UP4_CAGCAAATCAGAAAGGAGTG
• BKK38700 (sigY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGACCGTGATCCCCCCT,  downstream forward: _UP4_CAGCAAATCAGAAAGGAGTG

References

Reviews

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Other original publications

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The SigY regulon

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