gmuR

168

transcriptional repressor (GntR family) of the gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG operon

Locus: BSU_05850

Molecular weight: 27.23 kDa

Isoelectric point: 7.44

Protein length: 237 aa

Gene length: 714 bp

Function: regulation of glucomannan utilization

Product: transcriptional repressor (GntR family)

Essential: no

Synonyms: gmuR, ydhQ

Genomic Context

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List of homologs in different organisms, belongs to COG2188 (Galperin et al., 2021)

Gene

Coordinates: 630,170  630,883

The protein

Protein family

• GntR family of transcription factors

Domains

• HTH gntR-type domain (aa 1-69) (according to UniProt)

Structure

• 2WV0 (PDB) (the structure of NagR, 29% identity) PubMed
• O05509 (AlphaFold)

Paralogous protein(s)

• NagR, TreR, YmfC, GamR, YydK, FrlR

Expression and Regulation

Operons

• Genes: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG

  Description: PubMed

  Regulation

  • induced by cellobiose (GmuR) PubMed

  Regulatory mechanism

  • GmuR: repression, PubMed, in gmuR regulon
  • CcpA: repression, PubMed, in ccpA regulon
  • AbrB: activation, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C192 (ydhQ::erm), available at the NBRP B. subtilis, Japan
• BKE05850 (gmuR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGGTATCCTCCGGCAGC,  downstream forward: _UP4_TGATTGCGGAGACAACAAGG
• BKK05850 (gmuR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGGTATCCTCCGGCAGC,  downstream forward: _UP4_TGATTGCGGAGACAACAAGG

References

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