GlpK

• Description: glycerol kinase
  
  

Gene name	glpK
Synonyms
Essential	no
Product	glycerol kinase
Function	glycerol utilization
Gene expression levels in SubtiExpress: glpK
Interactions involving this protein in SubtInteract: GlpK
Metabolic function and regulation of this protein in SubtiPathways: glpK
MW, pI	54 kDa, 4.985
Gene length, protein length	1488 bp, 496 aa
Immediate neighbours	glpF, glpD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GlpP regulon

The gene

Basic information

• Locus tag: BSU09290

Database entries

• BsubCyc: BSU09290

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + glycerol = ADP + sn-glycerol 3-phosphate (according to Swiss-Prot)

• Protein family: FGGY kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PtsH-GlpK

• Localization:

Database entries

• BsubCyc: BSU09290

• Structure: 3H46 (from Enterococcus casseliflavus, complex with glycerol) PubMed

• UniProt: P18157

• KEGG entry: [3]

• E.C. number: 2.7.1.30

Expression and regulation

• Operon: glpF-glpK PubMed

• Expression browser: glpK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (8-fold) (CcpA) PubMed
  • induction by glycerol (GlpP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • GlpP-dependent RNA switch (transcriptional antitermination) PubMed
  • AbrB: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 151 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 6111 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 263 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 571 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1140 PubMed

Biological materials

• Mutant:
  • BKE09290 (glpK::ermC) (available at the BGSC and in Jörg Stülke's lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Josef Deutscher, Paris-Grignon, France