radC

radC
168

probable DNA repair protein

Locus
BSU_28040
Molecular weight
26.00 kDa
Isoelectric point
7.83
Protein length
Gene length
Function
DNA repair
Product
probable DNA repair protein
Essential
no
Synonyms
radC, ysxA

Genomic Context

List of homologs in different organisms, belongs to COG2003 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, SigM regulon, ComK regulon

Gene
Coordinates
2,861,840  2,862,535
The protein
Protein family
UPF0758 family (single member, according to UniProt)
MPN domain (aa 109-231) (according to UniProt)
Structure
2QLC (PDB) (C-terminal domain of Chlorobium tepidum RadC, 46% identity)
Modification
phosphorylated on Arg-59 PubMed
cytoplasm (Homogeneous) PubMed
Expression and Regulation
Operons
Description
Regulation
constitutively expressed PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
Sigma factors
SigM: sigma factor, promoter p2 within Maf PubMed, in sigM regulon
SigA: sigma factor, promoter p1, upstream of Maf PubMed, in sigA regulon
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2025-07-25 03:27:20

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5cb36570cb9eb74ac55107ce2bd7f07e7d2d5c0b

EE31E7A5D1B0C8BDC56B004AA68A1C721D4DA9CC

Biological materials
Mutant
GP3561 (ΔradC::aphA3), available in Jörg Stülke's lab
MGNA-B011 (ysxA::erm), available at the NBRP B. subtilis, Japan
BKE28040 (radC::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAGCACTCCTTTTCTTTCC,  downstream forward: _UP4_TAACACTTTTTTTTTCGTCG
BKK28040 (radC::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAGCACTCCTTTTCTTTCC,  downstream forward: _UP4_TAACACTTTTTTTTTCGTCG
References
Mirouze N, Ferret C, Yao Z, Chastanet A, Carballido-López R MreB-Dependent Inhibition of Cell Elongation during the Escape from Competence in Bacillus subtilis. PLoS genetics. 2015 Jun; 11(6):e1005299. doi:10.1371/journal.pgen.1005299. PMID:26091431
Zeigler DR Complete Genome Sequence of Bacillus subtilis Phage {varphi}105. Genome announcements. 2013 Sep 05; 1(5). doi:10.1128/genomeA.00641-13. pii:e00641-13. PMID:24009114
Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America. 2012 May 08; 109(19):7451-6. doi:10.1073/pnas.1117483109. PMID:22517742
Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science (New York, N.Y.). 2012 Mar 02; 335(6072):1103-6. doi:10.1126/science.1206848. PMID:22383849
Guariglia-Oropeza V, Helmann JD Bacillus subtilis σ(V) confers lysozyme resistance by activation of two cell wall modification pathways, peptidoglycan O-acetylation and D-alanylation of teichoic acids. Journal of bacteriology. 2011 Nov; 193(22):6223-32. doi:10.1128/JB.06023-11. PMID:21926231
Eiamphungporn W, Helmann JD The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses. Molecular microbiology. 2008 Feb; 67(4):830-48. doi:10.1111/j.1365-2958.2007.06090.x. PMID:18179421
Jervis AJ, Thackray PD, Houston CW, Horsburgh MJ, Moir A SigM-responsive genes of Bacillus subtilis and their promoters. Journal of bacteriology. 2007 Jun; 189(12):4534-8. . PMID:17434969
Meile JC, Wu LJ, Ehrlich SD, Errington J, Noirot P Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory. Proteomics. 2006 Apr; 6(7):2135-46. . PMID:16479537
Thackray PD, Moir A SigM, an extracytoplasmic function sigma factor of Bacillus subtilis, is activated in response to cell wall antibiotics, ethanol, heat, acid, and superoxide stress. Journal of bacteriology. 2003 Jun; 185(12):3491-8. . PMID:12775685
Ogura M, Yamaguchi H, Kobayashi K, Ogasawara N, Fujita Y, Tanaka T Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK. Journal of bacteriology. 2002 May; 184(9):2344-51. . PMID:11948146
Berka RM, Hahn J, Albano M, Draskovic I, Persuh M, Cui X, Sloma A, Widner W, Dubnau D Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK. Molecular microbiology. 2002 Mar; 43(5):1331-45. . PMID:11918817

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Time of last update: 2025-07-29 12:14:47

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