yesU

168

carbohydrate-binding protein, binds fucosylated glycans

Locus: BSU_07030

Molecular weight: 25.06 kDa

Isoelectric point: 7.71

Protein length: 220 aa

Gene length: 663 bp

Function: carbohydrate-binding protein

Product: unknown

Essential: no

Synonyms: yesU

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 768,828 → 769,490

The protein

Structure

• 1OQ1 (PDB)
• O31524 (AlphaFold)

Expression and Regulation

Operons

• Genes: rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ

  Description: PubMed

  Regulation

  • induced by pectin (RhgR, RhgL) PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • RhgR: activation, PubMed, in rhgR regulon
  • SigA: Sigma factor, PubMed, in sigA regulon
  • RhgL: activation, PubMed, in rhgL regulon
  • CcpA: repression, PubMed, in ccpA regulon

Biological materials

Mutant

• MGNA-B452 (yesU::erm), available at the NBRP B. subtilis, Japan
• BKE07030 (ΔyesU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTGCGGTACAAGCAAGCGC,  downstream forward: _UP4_GCCGTGCATCAAGCGGTGAG
• BKK07030 (ΔyesU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTGCGGTACAAGCAAGCGC,  downstream forward: _UP4_GCCGTGCATCAAGCGGTGAG

References

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