rhaA

168

L-rhamnose isomerase

Locus: BSU_31180

Molecular weight: 48.48 kDa

Isoelectric point: 5.35

Protein length: 424 aa

Gene length: 1275 bp

Function: rhamnose utilization

Product: L-rhamnose isomerase

Essential: no

E.C.: 5.3.1.14

Synonyms: rhaA, yulE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4806 (Galperin et al., 2021)

Gene

Coordinates: 3,197,933 → 3,199,207

The protein

Catalyzed reaction/ biological activity

• L-rhamnose = L-rhamnulose PubMed

Protein family

• rhamnose isomerase family (single member, according to UniProt)

Structure

• 3P14 (PDB) (from B. halodurans, 78% identity) PubMed
• O05264 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rhaEW-rhaR-rhaB-rhaM-rhaA

  Description: PubMed

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

  Regulatory mechanism

  • RhaR: repression, PubMed, in rhaR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: rhaR-rhaB-rhaM-rhaA

  Description: PubMed

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

Biological materials

Mutant

• MGNA-B544 (yulE::erm), available at the NBRP B. subtilis, Japan
• BKE31180 (ΔrhaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGGCTCTCTCCCTTTA,  downstream forward: _UP4_TAATAAAAAATCCGCCGCGG
• BKK31180 (ΔrhaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGGCTCTCTCCCTTTA,  downstream forward: _UP4_TAATAAAAAATCCGCCGCGG

References

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